A screen of random sequences for those that alter the trafficking of the influenza virus hemagglutinin in vivo |
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Authors: | Lewis C M Latham K Roth M G |
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Affiliation: | Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas TX 75235-9038, USA |
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Abstract: | In order to determine if the sequence patterns known to specify internalization represent the majority of possible internalization signals, we identified random sequences capable of causing a reporter protein to be internalized at least several-fold faster than the rate of non-selective internalization of membrane by clathrin-coated pits. A library of influenza hemagglutinin (HA) proteins, bearing short random sequences in place of the wild-type cytoplasmic domain, was prepared in recombinant SV40 virus. The library was expressed and screened for HAs that could internalize anti-HA antibody from the medium. The cytoplasmic sequences of the selected proteins were determined. From a small sample of sequences, we detected several that did not resemble those previously identified. The known internalization signals must represent only a subset of the sequences that can serve as internalization signals. |
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Keywords: | endocytosis influenza virus hemagglutinin internalization signals mutant screen protein traffic |
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