|
|||||
|
|
Inhibitory effects of epigallocatechin gallate and its glucoside on the human intestinal maltase inhibition |
|
| Authors: | Thi Thanh Hanh Nguyen Sun-Hwa Jung Sun Lee Hwa-Ja Ryu Hee-Kyoung Kang Young-Hwan Moon Young-Min Kim Atsuo Kimura Doman Kim |
| Affiliation: | 1. School of Biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University, Gwangju, 500-757, Korea 2. Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University, Gwangju, 500-757, Korea 3. Audubon Sugar Institute, LSU Ag Center, Baton Rouge, LA, 70803, USA 4. Eco-Friendly Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 580-185, Korea 5. Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan |
| Abstract: | Human intestinal maltase (HMA) is an ??-glucosidase responsible for the hydrolysis of ??-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA has become an important target in the treatment of type-2 diabetes. In this study, epigallocatechin gallate (EGCG) and EGCG glucoside (EGCG-G1) were identified as inhibitors of HMA by an in vitro assay with IC50 of 20 ± 1.0 and 31.5 ± 1.0 ??M, respectively. A Lineweaver-Burk plot confirmed that EGCG and EGCG-G1 were competitive inhibitors of maltose substrate against HMA and inhibition kinetic constants (K i ) calculated from a Dixon plot were 5.93 ± 0.26 and 7.88 ± 0.57 ??M, respectively. Both EGCG and EGCG-G1 bound to the active site of HMA with numerous hydrophobic and hydrogen bond interactions. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |