Cu(I)-thionein release from copper-loaded yeast cells |
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Authors: | Klaus Felix Hans-Jürgen Hartmann Ulrich Weser |
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Affiliation: | (1) Anorganische Biochemie, Physiologisch-Chemisches Institut der Universität Tübingen, Hoppe-Seyler Strasse 4, D-7400 Tübingen, Germany |
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Abstract: | Summary The release of intact CU(I)8-thionein from copper-resistant copper-loaded yeast cells, strain X 2180-1Aa, has been shown. This copper(I)-thiolate-rich protein was characterized and compared with the chemical and physicochemical properties of intracellular yeast Cu-thionein. The same molecular mass and stoichiometry of 8 mol copper atoms/mol protein was found. No detectable difference between the Cu-thioneins was seen in luminescence emission, electronic absorption in the ultraviolet region, chiroptical data or amino acid composition. The importance of stable Cu(I)-thiolates in Cu-thionein as a safe vehicle for transporting copper in a non-reactive manner is confirmed. |
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Keywords: | Cu(I)8-thionein Yeast Extracellular Circular dichroism Fluorescence Electronic absorption |
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