Construction of bifunctional fusion proteins consisting of duck BAFF and EGFP |
| |
Authors: | Wen-Bing Dan Chao Zhang Zheng-Bing Guan Shuang-Quan Zhang |
| |
Institution: | (1) Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210097, China |
| |
Abstract: | We constructed fusion proteins consisting of fluorescence-enhanced green fluorescent protein (EGFP) and soluble domain of
duck B-cell-activating factor of the TNF family (dsBAFF). The soluble EGFP/dsBAFF was efficiently expressed in Escherichia
coli BL 21 (DE3) and was purified in milligram amounts using metal chellate affinity chromatography. The fusion protein exhibited
similar fluorescence spectra with free EGFP and promoted the survival of duck bursal B cells in vitro as well as dsBAFF. EGFP/dsBAFF
has shown specific binding to duck BAFF receptors positive-cells and the stained cells could be analyzed with flow cytometry.
Thus, the fusion protein represents a readily obtainable source of biologically active dsBAFF that may prove useful in further
studies on duck BAFF and its receptors. |
| |
Keywords: | Duck B-cell-activating factor Fluoresce-enhanced green fluorescent protein Fusion protein Laser scanning confocal microscopy TNF receptor protein family |
本文献已被 PubMed SpringerLink 等数据库收录! |