| 副干酪乳杆菌HD1.7产生抗菌物质的初步研究 |
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| 引用本文： | 雷虹,李秀凉,庄海霁,张铁丹,周东坡,平文祥.副干酪乳杆菌HD1.7产生抗菌物质的初步研究[J].工业微生物,2007,37(4):16-19. |
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| 作者姓名： | 雷虹 李秀凉 庄海霁 张铁丹 周东坡 平文祥 |
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| 作者单位： | 1. 黑龙江大学,生命科学学院,微生物黑龙江省高校重点实验室,哈尔滨,150080;哈尔滨医科大学,公共卫生学院,营养与食品卫生国家重点学科,哈尔滨,150080
2. 黑龙江大学,生命科学学院,微生物黑龙江省高校重点实验室,哈尔滨,150080 |
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| 基金项目： | 黑龙江省科技厅重大项目(GB05B401)，黑龙江省发改委重大攻关课题(项目号[2002]1199)，哈尔滨市青年科学研究基金项目(2003AFQXJ015) |
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| 摘 要： | 对从乳酸菌酸菜发酵液中分离到的能产生抑菌物质的菌株进行了鉴定,并对该菌的发酵产物进行了提取和性质研究。经形态学、生理生化与16SrDNA序列分析鉴定该菌株为副干酪乳杆菌(Lactobacillus paracasei)。抑菌谱试验表明:除酵母菌外,该抑菌物质对革兰氏阳性、阴性菌和多种致病菌均有较强的抑制作用。经有机溶剂萃取法获得了抑菌物质的粗提物,胰蛋白酶水解证明抑菌物质具有蛋白质性质,尿素-SDS-PAGE不连续凝胶电泳法测得抑菌物质的分子量为14000左右。
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| 关 键 词： | 副干酪乳杆菌 抑菌谱 抑菌物质 提取 蛋白质性质 |
Studies on antimicrobial substance produced by Lb. paracasei HD1.7 |
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| LEI Hong,LI Xiu-liang,ZHUANG Hai-ji,ZHANG Tie-dan,ZHOU Dong-po,PING Wen-xiang.Studies on antimicrobial substance produced by Lb. paracasei HD1.7[J].Industrial Microbiology,2007,37(4):16-19. |
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| Authors: | LEI Hong LI Xiu-liang ZHUANG Hai-ji ZHANG Tie-dan ZHOU Dong-po PING Wen-xiang |
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| Institution: | 1. Education Department Key Microbiology Lab, College of Life Science, Heilongiiang University, Harbin 150080, China; 2. The State Key Subject of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin 150080, China |
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| Abstract: | A strain producing antimicrobial substance was identified.Its product was separated and its properties were studied.The strain was identified as Lactobacillus paracasei based on the morphologic characteristics,physiologic-biochemical characteristics and the analysis of 16SrDNA gene sequence.The antimicrobial spectrum tests indicated that the antimicrobial substance had strong inhibitory activity against gram-positive bacteria and gram-negative bacteria,and also against various pathogens.Extracted with organic solvent,the crude extract were obtained.The antimicrobial substance could be hydrolyzed by trysin.The molecular weight of the antimicrobial substance was about 14000 determined by the Urea-SDS-Polyacrylamide Gel Electrophoresis technique. |
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| Keywords: | Lactobacillus paracasei antimicrobial spectrum antimicrobial substance extraction proteinaceous characteristics |
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