| 猪卵母细胞体外成熟和体外受精卵表面元素的研究 |
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| 引用本文： | 冯怀亮,秦鹏春.猪卵母细胞体外成熟和体外受精卵表面元素的研究[J].生物技术,1991,1(3):12-15. |
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| 作者姓名： | 冯怀亮 秦鹏春 |
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| 作者单位： | 东北农学院生物工程系 哈尔滨市
(冯怀亮，秦鹏春，吴光明)，东北农学院生物工程系 哈尔滨市(谭景和) |
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| 基金项目： | 国家农业部七五资助课题 |
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| 摘 要： | 本文应用X-射线能量色散技术对猪卵泡内卵母细胞,培养不同时间卵母细胞和体外受精卵进行了表面元素的定性和定量分析,结果表明,(1)在卵母细胞和受精卵的表面均含有Na、Mg、Al、Si、S、Cl、K、Ca和Fe元素;(2)A级卵母细胞随着体外培养,Ca的含量逐渐升高,而K的含量逐渐降低;(3)B、C级卵母细胞随着其培养Al、Ca~(2+)降低;(4)受精后卵表面的Ca显著升高。
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| 关 键 词： | 猪 卵母细胞 体外受精 元素 |
STUDY ON THE SURFACE OF FELEMENT CONSTITUENCY OF PIG OOCYIES MATURED AND FERTILIZED IN VITRO |
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| Feng Huailiang Qin Pengchun Wu Guangming Tan Jinghe.STUDY ON THE SURFACE OF FELEMENT CONSTITUENCY OF PIG OOCYIES MATURED AND FERTILIZED IN VITRO[J].Biotechnology,1991,1(3):12-15. |
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| Authors: | Feng Huailiang Qin Pengchun Wu Guangming Tan Jinghe |
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| Abstract: | With the X-ray energy chromatic dispersion technique.the surface elements of pig oocytes and external fertilized eggs were analysed qualitatively and quantitatively. The results showed as follows: (1 )The surface of the oocytes and fertilized eggs contained Na.Mg,Al,Si,S.Cl,K,Ca and Fe; (2)As the oocytes matured progress,their Ca content increased(P<0. 05) ;their K content decreased(P<0. 05); (3)After maturation theirr Al, Ca contents decreasde significantly(P<0. 01); (4)After fertilization ,the Ca content increased significant-ly(P<0. 01). |
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| Keywords: | pig oocytes external fertilization elements |
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