Isolation and purification of hog renin |
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| Authors: | HAAS E LAMFROM H GOLDBLATT H |
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| Affiliation: | 1. Department of Chemistry and Biochemistry, South Dakota State University, Box 2202, Brookings, SD 57007, USA;2. Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA 92612, USA;3. Analytical Toxicology Division, United States Army Medical Research Institute of Chemical Defense, 3100 Ricketts Point Road, Aberdeen Proving Ground, MD 21010, USA;1. Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;2. Department of Chemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge CB3 0AS, United Kingdom |
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| Abstract: | - 1.1. A new procedure has been described for the isolation and fractionation of renin on a pilot plant scale.
- 2.2. Renin has been purified 56,000-fold in the ten fractionation steps of this procedure, and it has a specific activity about 40 times greater than that of renin preparations previously described.
- 3.3. Optimum conditions have been established for the quantitative recovery of renin in each of these steps, for the separation of material capable of producing anaphylaxis, and for the storage of renin.
- 4.4. Seasonal variations of several hundred per cent were observed in the renin content of hog kidneys, sufficient in magnitude to require consideration in the preparation of renin on a large scale.
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