Lymphocyte responses of human neonates to bacterial antigens |
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Authors: | H R Rubin R U Sorensen S H Polmar |
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Institution: | 1. Department of Tumor Biology, Karolinska Institute, Sweden;2. Department of Endocrinology, Karolinska Hospital, S-104 01 Stockholm, Sweden |
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Abstract: | Human lymphoid lines derived from normal or neoplastic B cells were assayed for insulin binding. 125I-Labeled insulin was allowed to bind to cells. Bound radioactivity which was inhibited with unlabeled insulin was regarded as specific binding. Among 46 lines tested, 43 bound more insulin than normal peripheral B lymphocytes. The majority of the lines resembled activated lymphocytes, with regard to their insulin binding. More mature cells represented by EBV-transformed lines of normal origin, bound more insulin than the less differentiated Burkitt lymphoma lines. However, even the latter bound significantly more insulin than peripheral blood lymphocytes. |
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Keywords: | To whom correspondence should be addressed at: Institute of Immunology Medical Sciences Bldg University of Toronto Toronto Ontario M5S 1A8 Canada |
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