Multiple forms of immunoreactive dynorphin in rat pituitary and brain |
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Authors: | T Suda F Tozawa S Tachibana H Demura K Shizume |
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Affiliation: | 7. Department of Medicine, Tokyo Women''s Medical College, 10-Kawada-Cho, Ichigaya, Shinjuku-Ku, Tokyo, Japan;71. Research Laboratory of Biochemistry, Eisai Co., Ltd., 4-Koishikawa, Bunkyo-Ku, Tokyo, Japan |
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Abstract: | Multiple forms of immunoreactive dynorphin (I-dynorphin) in rat anterior pituitary (AP), intermediate-posterior pituitary (IP) and medial basal hypothalamus (MBH) were examined. Three I-dynorphin peaks were observed on gel filtration. The first peak (big form) was eluted near the position of beta-LPH, and was predominant in AP. The second peak (middle form) was eluted near the position of ACTH. The third peak (small form) was eluted at the position of dynorphin (1-13), ane was predominant in IP and MBH. The heterogeneity of this small form was examined by ion exchange chromatography and reverse phase high performance liquified chromatography (HPLC). I-dynorphin peaks were observed at the positions of dynorphin(1-17), (1-13), (1-11), (1-10) and other peptides. These results strongly suggest (i) the presence of dynorphin(1-17), (1-13), (1-11) and (1-10) in rat IP, (ii) dynorphin(1-11) and (1-10) as the major components in this small form, (iii) the difference of I-dynorphin processing in AP, IP and MBH. |
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Keywords: | To whom all correspondence should be directed. |
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