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Synthesis and purification of [1-32p]fructose-1,6-bisophosphate with high specific radioactivity.
Authors:R W McClard
Institution:Department of Chemistry, University of California, Los Angeles, California 90024 USA
Abstract:The straightforward, rapid synthesis and purification of radiochemically pure 1-32P]fructose-1,6-bisphosphate starting from radioactively labeled inorganic phosphate is described. The product has a relatively high specific radioactivity (2 × 105 Ci/mol). Using this method, carrier-free 1-32P]fructose-1,6-bisphosphate could be obtained. The use of 1-32P]fructose-1,6-bisphosphate in the assay of fructose bisphosphatase is illustrated.
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