Synthesis and purification of [1-32p]fructose-1,6-bisophosphate with high specific radioactivity.

The straightforward, rapid synthesis and purification of radiochemically pure 1-³²P]fructose-1,6-bisphosphate starting from radioactively labeled inorganic phosphate is described. The product has a relatively high specific radioactivity (2 × 10⁵ Ci/mol). Using this method, carrier-free 1-³²P]fructose-1,6-bisphosphate could be obtained. The use of 1-³²P]fructose-1,6-bisphosphate in the assay of fructose bisphosphatase is illustrated.