Anticancer activity in vitro of a fucoidan from the brown alga Fucus evanescens and its low-molecular fragments, structurally characterized by tandem mass-spectrometry |
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Authors: | Stanislav D. Anastyuk Natalia M. ShevchenkoSvetlana P. Ermakova Olesya S. VishchukEvgeny L. Nazarenko Pavel S. DmitrenokTatyana N. Zvyagintseva |
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Affiliation: | Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, 100 Let Vladivostok Ave. 159, 690022 Vladivostok, Russian Federation |
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Abstract: | Fucoidan was isolated and purified from the brown algae Fucus evanescens and subjected to autohydrolysis to obtain low-molecular-weight fragments. MALDI-TOFMS analysis has shown that monosulfated fucose and more heavily sulfated (up to 5) fucooligosaccharides with polymerization degree (DP) 2, 4 and 6, including galactose-containing sulfated oligosaccharides were the products of autohydrolysis. The structural features of these fragments were elucidated by negative-ion potential lift tandem MALDI-TOF mass-spectrometry using arabinoosazone as a matrix, which allowed reducing the in-source fragmentation. Native fucoidan has been shown to exert anticancer activities in both human malignant melanoma cell lines SK-MEL-28 and SK-MEL-5. Low-molecular-weight fragments exhibited almost no action to cell proliferation in both cell lines and colony formation on SK-MEL-5 cells, but its inhibition activity to the colony formation of SK-MEL-28 cell lines was as high as was demonstrated by native fucoidan (70%). Probably, the inhibiting activity for SK-MEL-28 depended on the presence of sulfates and (1 → 4)-linked α-l-Fucp residues in the main chain of fucoidan/oligosaccharides. |
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Keywords: | Fucoidan derivatives Structure MS/MS Anticancer activity |
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