N-glycosylated variants of growth hormone in human pituitary extracts |
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Authors: | García-Barros M Costoya J A Ríos R Arce V Devesa J |
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Institution: | Departamento de Fisiología, Facultad de Medicina, Santiago de Compostela, Espa?a. |
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Abstract: | This study was designed to investigate the existence, in human pituitary extracts, of growth hormone (GH) variants not encoded by the hGH-N gene. Using anion exchange-fast protein liquid chromatography followed by SDS-PAGE, we isolated several basic forms of pituitary GH. Incubation of these basic forms with endoglycosidase F/N-glycosidase F revealed that two of them (about 34 and 12 kD) were N-glycosylated. In contrast, no changes were found when samples were incubated with the O-linked glycosylation-specific O-glycosidase. Since the GH-N molecule lacks consensus sequences for N-linked glycosylation, our findings suggest that GH genes other than hGH-N are expressed in the human pituitary gland. |
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