一种在两栖类胚胎的免疫组织化学研究中有多种用途的方法

本文介绍了我们最近发展的一项用于两栖类胚胎的免疫组织化学研究的技术。两栖类胚胎经过适当的化学固定以后,用振动切片机可以得到50—100 μ的切片。我们用这样的切片进行免疫萤光和免疫酶标染色,均得到满意的结果,可以进行光镜(共聚焦显微镜,普通显微镜)及透射电镜的观察。由于在整个过程中避免了使用有机溶剂及包埋剂,所以最大限度地保存了抗原性。与传统的各种免疫组化技术比较,切片的各部分组织均能迅速与抗体反应,组织保存相当完好,可以满足电镜观察的要求。运用这种方法,还可以将同一胚胎的不同切片分别用于光镜和电镜观察,使结果更具说服力。

A VERSATILE METHOD FOR THE IMMUNOHISTOLOGICAL ANALYSIS OF AMPHIBIAN EMBRYOS

The immunostaining of vibratome sec-tions taken from fixed embryos is a ver-satile immunohistological method overcom-ing some of the problems experienced byconventional techniques.All embryonic re-gions are easily accessible to antibodies;as no extracting agents are applied thestaining pattern is believed to mirror thein vivo antigen distribution quite closely;tissue preservation is of high quality andsuitable for TEM studies:stained slicesfrom one and the same embryo can beinspected by means of the dissecting mi-croscope,the confocal laser scan micro-scope,the light microscope,and the trans-mission electron microscope.