Charcoal adsorption assay for measurement of colchicine binding and tubulin content of crude tissue extracts. |
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| Authors: | E Rapaport P D Berkley N L Bucher |
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| Institution: | The John Collins Warren Laboratories of the Huntington Memorial Hospital of Harvard University at the Massachusetts General Hospital, Boston, Massachusetts 02114 USA |
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| Abstract: | A rapid, reproducible, and quantitative colchicine-binding assay for tubulin content of crude tissue extracts is described and applied to the high speed supernatant fraction of rat liver homogenates. Utilizing Scatchard plots, the colchicine-tubulin association constant is found to be in general agreement with values reported for purified tubulin from other sources. |
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