Biosynthesis of hyperforin and adhyperforin from amino acid precursors in shoot cultures of Hypericum perforatum |
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Authors: | Karppinen Katja Hokkanen Juho Tolonen Ari Mattila Sampo Hohtola Anja |
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Affiliation: | Department of Biology, University of Oulu, P.O. Box 3000, FIN-90014 Oulu, Finland. katja.karppinen@oulu.fi |
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Abstract: | Hyperforin and adhyperforin contribute to the antidepressant effects of Hypericum perforatum. The involvement of branched-chain amino acids in the biosynthesis of hyperforin and adhyperforin was demonstrated in H. perforatum shoot cultures. L-[U-(13)C(5)]Valine and L-[U-(13)C(6)]isoleucine, upon administration to the shoot cultures, were incorporated into acyl side chain of hyperforin and adhyperforin, respectively. Feeding the shoot cultures with unlabelled L-isoleucine at a concentration of 2mM induced a 3.7-fold increase in the production of adhyperforin. The addition of 3mM L-threonine, a precursor of isoleucine, stimulated a 2.0-fold increase in the accumulation of adhyperforin. The administration of L-valine at concentrations of 0-5mM had no stimulating effect on the hyperforin production in H. perforatum shoot cultures. |
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Keywords: | Hypericum perforatum Biosynthesis Shoot culture Adhyperforin Hyperforin Isoleucine Threonine Valine |
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