Lysophosphatidylserine stimulates leukemic cells but not normal leukocytes |
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Authors: | Park Kyoung Sun Lee Ha Young Kim Mi-Kyoung Shin Eun Ha Bae Yoe-Sik |
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Institution: | Medical Research Center for Cancer Molecular Therapy, College of Medicine, Dong-A University, Busan 602-714, Republic of Korea. |
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Abstract: | In this study, we observed that lysophosphatidylserine (LPS) stimulated intracellular calcium (Ca(2+)](i)) increase in leukemic cells but not in normal human peripheral blood mononuclear cells. LPS also stimulated Ca(2+)](i) increase in human leukemic THP-1 cells. LPS-stimulated Ca(2+)](i) increase was inhibited by U-73122 but not by U-73343. LPS also stimulated inositol phosphates formation in THP-1 cells, suggesting that LPS stimulates calcium signaling via phospholipase C activation. Moreover, pertussis toxin (PTX) completely inhibited Ca(2+)](i) increase by LPS, indicating the activation of PTX-sensitive G-proteins. We also found that LPS-induced Ca(2+)](i) increase was completely inhibited by suramin, suggesting G-protein coupled receptor activation. Since LPS specifically stimulates PTX-sensitive G-proteins, phospholipase C-dependent Ca(2+)](i) increase in leukemic cells but not normal peripheral blood leukocytes, LPS receptor may be associated with leukemia. |
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Keywords: | Lysophosphatidylserine Intracellular calcium increase Leukemic cells Pertussis toxin-sensitive G-protein |
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