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反向核不育水稻FHS组织培养高频率植株再生
引用本文:王松丽,黄群策,秦广雍,.反向核不育水稻FHS组织培养高频率植株再生[J].广西植物,2006,26(3):278-281.
作者姓名:王松丽  黄群策  秦广雍  
作者单位:郑州大学,离子束生物工程省重点实验室,河南,郑州,450052
摘    要:以反向核不育水稻品系FHS为研究材料,对其成熟胚愈伤组织的诱导及其再生条件进行了研究。研究结果表明,通过选择合适的培养基和调节2,4-D的浓度,胚性愈伤组织的诱导率可以达到92.5%。通过调节MS培养基中KT和NAA的浓度,并加入适量的CuSO4,植株再生频率高达81.3%。由此可见,合适的激素浓度的高低与FHS植株再生频率的高低呈现出一定的相关性。

关 键 词:反向核不育水稻  组织培养  愈伤组织  诱导  植株再
文章编号:1000-3142(2006)03-0278-04
收稿时间:2005-06-13
修稿时间:2005-12-09

High frequency of plantlet regeneration from revers genic sterile rice FHS tissue culture
WANG Song-li,HUANG Qun-ce,QIN Guang-yong.High frequency of plantlet regeneration from revers genic sterile rice FHS tissue culture[J].Guihaia,2006,26(3):278-281.
Authors:WANG Song-li  HUANG Qun-ce  QIN Guang-yong
Institution:Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou 450052, China
Abstract:The culture condition of callus formation and plantlet regeneration of revers genic sterile rice variety FHS was studied. By selecting suitable medium and adjusting the concentration of 2,4-D,the induction frequency of calli reached 92.5%;After adjusting the concentration of KT and NAA,the rate of calli differentiation was 81.3%. The result showed that the suitable concentration of phytohormone could promote the efficiency of FHS plantlet regeneration.
Keywords:revers genic sterile rice  tissue culture  callus  induction  plantlet regeneration
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