Osmolyte contents of cultured astrocytes grown in hypoosmotic medium |
| |
Institution: | Department of Emergency Medicine, Department of Physiology and Biophysics, Wright State University School of Medicine, Cox Institute, 3525 Southern Boulevard, Kettering, OH 45429, USA |
| |
Abstract: | Primary rat cerebral astrocyte cultures were grown for 2 weeks in isoosmotic medium (305 mosmol) and then placed in similar medium with a reduced NaCl concentration. During the first hour of growth in this moderately hypoosmotic medium (240 mosmol), the cells lose 88% of their taurine contents, 62% of their alanine contents, and 54% of their aspartate contents while regaining normal volume. Loss of these amino acids accounts for 43% of observed volume regulation. Contents of these amino acids remain decreased during 24 h of growth in hypoosmotic medium. In contrast, potassium, glutamate, glutamine, and asparagine contents are not changed, relative to cells in isoosmotic medium, at time points between 1 h and 24 h of hypoosmotic exposure. The data suggest astrocytes contribute to net loss of amino acids, but not potassium, from brains exposed to hypoosmotic conditions in situ. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|