RAD51 supports DMC1 by inhibiting the SMC5/6 complex during meiosis |
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| Authors: | Hanchen Chen Chengpeng He Chongyang Wang Xuanpeng Wang Fengyin Ruan Junjie Yan Ping Yin Yingxiang Wang Shunping Yan |
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| Affiliation: | 1. College of Life Science and Technology, Center of Integrative Biology, Interdisciplinary Science Research Institute, Huazhong Agricultural University, Wuhan 430070, China;2. State Key Laboratory of Genetic Engineering and Ministry of Education, Key Laboratory of Biodiversity Sciences and Ecological Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China |
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| Abstract: | Meiosis is a fundamental process for sexual reproduction in most eukaryotes and the evolutionarily conserved recombinases RADiation sensitive51 (RAD51) and Disrupted Meiotic cDNA1 (DMC1) are essential for meiosis and thus fertility. The mitotic function of RAD51 is clear, but the meiotic function of RAD51 remains largely unknown. Here we show that RAD51 functions as an interacting protein to restrain the Structural Maintenance of Chromosomes5/6 (SMC5/6) complex from inhibiting DMC1. We unexpectedly found that loss of the SMC5/6 partially suppresses the rad51 knockout mutant in terms of sterility, pollen inviability, and meiotic chromosome fragmentation in a DMC1-dependent manner in Arabidopsis thaliana. Biochemical and cytological studies revealed that the DMC1 localization in meiotic chromosomes is inhibited by the SMC5/6 complex, which is attenuated by RAD51 through physical interactions. This study not only identified the long-sought-after function of RAD51 in meiosis but also discovered the inhibition of SMC5/6 on DMC1 as a control mechanism during meiotic recombination. RAD51 functions as an interacting protein to restrain the SMC5/6 complex from inhibiting DMC1 during meiosis. |
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