| 牙龈卟啉菌内毒素的化学组成和SDS—PAGE的特性分析 |
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| 引用本文： | 陈莉明,严杰,倪可夫.牙龈卟啉菌内毒素的化学组成和SDS—PAGE的特性分析[J].中国微生态学杂志,1999,11(4):222-224. |
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| 作者姓名： | 陈莉明 严杰 倪可夫 |
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| 作者单位： | 浙江医科大学附属二院口腔科(陈莉丽)，浙江医科大学微生物学教研室(严杰)，杭州市第三人民医院口腔科(倪可夫) |
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| 摘 要： | 应用气液色谱法对牙龈卟啉菌脂多糖（Ｐｇ－ＬＰＳ）和大肠杆菌脂多糖（Ｅ－ＬＰＳ）的单糖和脂肪酸进行了分析和比较。Ｐｇ－ＬＰＳ主要含有甘露糖、葡萄糖、氨基葡萄糖、半乳糖和鼠李糖。Ｅ－ＬＰＳ单糖组成与Ｐｇ－ＬＰＳ基本相似，但缺乏鼠李糖。Ｐｇ－ＬＰＳ主要含有羟基十五烷酸和羟基十七烷酸等奇数碳原子脂肪酸，Ｅ－ＬＰＳ则以羟基豆蔻酸、豆蔻酸和月桂酸等偶数碳原子脂肪酸为主。Ｐｇ－ＬＰＳ含有甲基化脂肪酸，Ｅ－ＬＰＳ则缺如。实验结果提示，Ｐｇ－ＬＰＳ和Ｅ－ＬＰＳ化学组成上的差异可能导致二者的ＳＤＳ－ＰＡＧＥ图谱明显不同。
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| 关 键 词： | 牙龈卟啉菌／内毒素 化学组成 SDS-PAGE |
| 修稿时间： | 1998-11-25 |
ANALYSES ON THE CHARACTERISTICS OF CHEMICAL COMPOSITIONS AND SDS-PAGE OF LIPOPOLYSACCHRIDE FROM PORPHYROMONAS GINGIVALIS |
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| Chen Li-li,et al..ANALYSES ON THE CHARACTERISTICS OF CHEMICAL COMPOSITIONS AND SDS-PAGE OF LIPOPOLYSACCHRIDE FROM PORPHYROMONAS GINGIVALIS[J].Chinese Journal of Microecology,1999,11(4):222-224. |
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| Authors: | Chen Li-li |
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| Institution: | Chen Li-li,et al.Department of Stomatology,the second affiliated hospital,Zhejiang Medical University,Hangshou 310009 |
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| Abstract: | The monosacchroses and fatty acids of lipopolysacchrides from porphyromonas gingivalis(Pg-LPS) and E.Coli(E-LPS) were analysed and compared by gas-liquid chromatography.The major monosacchroses in Pg-LPS were mannose,glucose,aminoglucose,galatose and rhamnose.The monosacchride composition of E-LPS was similar to that of Pg-LPS but lack of rhamnose.Pg-LPS contained mainly fatty acids with odd carbon atoms such as pentadecoic acid and heptadecoic acid,while E-LPS possessed fatty acids with even carbon atoms of hydroxy myritic acid,myritic acid and lauxic acid.Methylated fatty acid was found in Pg-LPS but not in E-LPS.The results showed that SDS-PAGE atlas of Pg-LPS was not similar to that of E-LPS because of difference of their chemical compositions. |
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| Keywords: | Porphyromonas gingivalis SPS-PAGE |
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