Molecular characterization of a LMW-GS gene located on chromosome 1B and the development of primers specific for the Glu-B3 complex locus in durum wheat |
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Authors: | R D’Ovidio M Simeone S Masci E Porceddu |
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Institution: | (1) Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy Fax: +39 761 357242 E-mail: dovidio@unitus.it, IT |
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Abstract: | Low-molecular-weight glutenin subunits (LMW-GS) represent a specific class of wheat storage proteins encoded at the Glu-3 loci. Particularly interesting are the LMW-GS encoded at the Glu-B3 locus because they have been shown to play an important role in determining the pasta-making properties of durum wheat. Genes
encoding LMW-GS have been characterized but only a few of them have been assigned to specific loci. Notably, no complete LMW-GS
gene encoded at the Glu-B3 locus has yet been described. The present paper reports the isolation and characterization of a lmw-gs gene located at the Glu-B3 locus. The clone involved, designated pLDNLMW1B, contains the entire coding region and 524 bp of the 5′ upstream region.
A nucleotide comparison between the pLDNLMW1B clone and other LMW-GS genes showed the presence of some peculiar structural
characteristics, such as short insertions in the promoter region, the presence of a cysteine codon in the repetitive domain,
and a more regular structure of this region, which could be important for its tissue-specific expression and for the functional
properties of the encoded protein, respectively.
Received : 30 May 1997 / Accepted : 29 July 1997 |
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Keywords: | Triticum durum LMW glutenin gene PCR Glu-B3 locus |
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