Chromosome painting of Y chromosomes and isolation of a Y chromosome-specific repetitive sequence in the dioecious plant Rumex acetosa |
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Authors: | F Shibata M Hizume Y Kuroki |
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Institution: | (1) Institute of Biology, Faculty of Science, Ehime University, Matsuyama 790-8577, Japan, JP;(2) Institute of Biology, Faculty of Education, Ehime University, Matsuyama 790-8577, Japan, JP |
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Abstract: | The dioecious plant Rumex acetosa has a multiple sex chromosome system: XX in female and XY1Y2 in male. Both types of Y chromosome were isolated from chromosome spreads of males by manual microdissection, and their chromosomal
DNA was amplified using degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR). When the biotin-labeled DOP-PCR
product was hybridized with competitor DNA in situ, the fluorescent signal painted the Y chromosomes. A library of Y chromosome
DNA was constructed from the DOP-PCR product and screened for DNA sequences specific to the Y chromosome. One Y chromosome-specific
DNA sequence was identified and designated RAYSI (R. acetosa Y chromosome-specific sequence I). RAYSI is a tandemly arranged repetitive DNA sequence that maps to the 4’,6-diamidino-2-phenylindole bands of both Y chromosomes.
Received: 22 December 1998; in revised form: 22 March 1999 / Accepted: 23 March 1999 |
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