| Napsin A基因对A549细胞在上皮-间质转化中增殖的影响及其机制 |
| |
| 引用本文： | 管淑红,郑金旭,许清,汤艳,刘继柱,吕晓婷.Napsin A基因对A549细胞在上皮-间质转化中增殖的影响及其机制[J].细胞生物学杂志,2011(2). |
| |
| 作者姓名： | 管淑红 郑金旭 许清 汤艳 刘继柱 吕晓婷 |
| |
| 作者单位： | 江苏大学基础医学与医学技术学院;江苏大学附属医院呼吸科; |
| |
| 基金项目： | 卫生部科研项目(No.wkj2006-2-026); 江苏省“333工程”(苏人才办2007-16-09)资助项目~~ |
| |
| 摘 要： | 采用慢病毒载体质粒PLJM1将NapsinA基因转染到人肺腺癌细胞——A549细胞中,获得稳定表达Napsin A蛋白的特性并鉴定,通过转化生长因子-β1刺激A549细胞发生上皮-间质转化,体外构建上皮-间质转化模型并鉴定。MTT法检测转基因前后A549细胞在上皮-间质转化过程中生长速率的变化;流式细胞术检测其细胞周期的改变,最后予Western blot检测黏着斑激酶的表达情况,探讨Napsin A基因对A549细胞在上皮-间质转化过程中增殖的影响及其机制。结果表明转染后的A549细胞表达Napsin A蛋白明显增加(P<0.01);A549细胞发生上皮-间质转化后细胞E钙蛋白表达下调(P<0.01),Ⅰ型胶原表达上调(P<0.01);转基因细胞在体外上皮-间质转化模型中增殖速度减慢(P<0.05),且细胞周期被阻滞在G_1期(P<0.01),其表达整合素信号传导通路的基础分子——黏着斑激酶的量显著下降(P<0.01)。提示Napsin A基因可以抑制A549细胞在上皮-间质转化过程中的进一步增殖,其机制可能与抑制整合素信号传导通路有关。
|
| 关 键 词： | Napsin A A549细胞 上皮-间质转化 增殖 黏着斑激酶 |
The Effect of Napsin A Gene on the Proliferation in Ephethlial-Mesenchymal Transition of Cell A549 and Its Mechanism |
| |
| Shu-Hong Guan,Jin-Xu Zheng,Qing Xu,Yan Tang,Ji-Zhu Liu,Xiao-Ting L.The Effect of Napsin A Gene on the Proliferation in Ephethlial-Mesenchymal Transition of Cell A549 and Its Mechanism[J].Chinese Journal of Cell Biology,2011(2). |
| |
| Authors: | Shu-Hong Guan Jin-Xu Zheng Qing Xu Yan Tang Ji-Zhu Liu Xiao-Ting L |
| |
| Institution: | Shu-Hong Guan~1,Jin-Xu Zheng~(2*),Qing Xu~2,Yan Tang~2,Ji-Zhu Liu~2,Xiao-Ting L(u|¨)~2 (1 School of Medical Science and Laboratory Medicine,Jiangsu University,Zhenjiang 212013,China,2 Department of Respiration,the Affiliated Hospital of Jiangsu University,Zhenjiang 212001,China) |
| |
| Abstract: | A recombinant lentiviral plasmid PLJM1-Napsin A was constructed and transfected into lung adenocarcinoma cell A549.Western blot assay was used to identify the stable expression of Napsin A protein. Ephethlial-mesenchymal transition model was established in vitro by the A549 cells treated with transforming growth factor beta-1.The cell proliferation was detected by MTT assay.The alteration of cell cycle were determined by flow cytometry.Then the expression of focal adhesion kinase were determined by Western ... |
| |
| Keywords: | Napsin A A549 cell ephethlial-mesenchymal transition proliferation focal adhesion kinase |
| 本文献已被 CNKI 等数据库收录！ |
|
