低温对沙土鼠脑缺血后微管运动蛋白和结构蛋白活性的影响

目的：研究低温对脑缺血后沙土鼠微管运动蛋白(Kinesin）微管结构蛋白（microtubule associated protein 2,MAP2）活性的影响,并探讨二者活性变化与延迟性神经元死亡（delayed neuronal death,DND）的关系。方法：Kinesin和MAP2的活性应用免疫组织化学染色结合计算机图象分析的方法测定,DND应用病理检查方法判断。结果：低温明显减少脑缺血后的DND。前脑缺血再灌注后MAP2和kinesin活性随再灌注时间延长而进行性下降,且kinesin活性下降程度大于MAP2。低温明显减少脑缺血后MAP2和kinesin活性的下降程度。Kinesin活性下降的严重程度与脑缺血后DND的严重程度相一致。结论：低温可明显减少脑缺血后的DND,其机制与其减少脑缺血后运动蛋白kine-sin活性的下降有关。

Effect of hypothermia on activities of tubule motor protein and constructure protein after cerebral ischemia in gerbils

Aim: To study the effect of hypothermia on kinesin and MAP2 activities after cerebral ischemia in gerbils, and investigate the relationship between changes of their activities and DND. Methods: Immunoreactivity of kinesin and MAP2 were determined by immunohistochemical study in combination with computer image analysis system, and DND were assayed by histological examination. Results: Hypothermia significantly reduced DND after cerebral ischemia. Immunoreactivities of MAP2 and kinesin progressively decreased during forebrain ischemia reperfusion, and the decreasing of kinesin activity was greater than that of MAP2 in the same reperfusion time.Hypothermia obviously mitigate the decreasing degree of MAP2 and kinesin activity after cerebral ischemia. The decreasing degree of kinesin activity was consistent with that of DND during cerebral ischemia/reperfusion in gerbils. Conclusion: Hypothermia significantly reduced DND after cerebral ischemia, which might be related to the improvement of kinesin activity after cerebral ischemia reperfuision in gerbils.