Ovulation response to pregnant mares' serum gonadotrophin in prepubertal ewe lambs of different Booroola genotypes |
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Authors: | GH Davis PD Johnstone |
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Institution: | Invermay Agricultural Research Centre, Ministry of Agriculture and Fisheries, Private Bag, Mosgiel New Zealand |
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Abstract: | Ovulation rates following treatment with PMSG were measured in prepubertal ewe lambs of known Booroola genotype to evaluate the technique as a method of early identification of Booroola genotypes. Two experiments were conducted with homozygous (FF), heterozygous (F+) and non-carrier (++) interbred Merino Romney breed type ewe lambs and a third experiment compared first cross F+ Merino Coopworth breed type with contemporary Coopworths (++). The ewe lambs aged 5–6 months were injected with 400 or 600 i.u. PMSG. The percentage of ewe lambs ovulating following treatment with PMSG was similar in all experiments (75–77%) but in Experiment 1 the FF and F+ lambs had a significantly higher proportion ovulating than the ++ lambs (P<0.05). The mean ovulation rate of FF ewe lambs treated with PMSG was 1.03 (Expt 1) and 1.69 (Expt 2) higher than ++ lambs (P<0.05). In Experiment 1 the mean ovulation rate of F+ ewe lambs was 0.33 higher than ++ ewe lambs but this difference was not significant. However, in Experiments 2 and 3 the mean ovulation rate of F+ ewe lambs treated with 600 i.u. PMSG was 1.06 and 0.45 higher than ++ ewe lambs respectively (P< 0.05). These results show that PMSG treatment is a promising technique for identifying the Booroola genotype of ewe lambs at an early age but further experiments with different dose rates of PMSG and different ages and liveweights of lambs are required to determine optimum dose rates and time of treatment. |
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