In Memoriam

Thirteen cows, Experiment I, were injected at 1 h post-partum with saline (n=4) or Human Chorionic Gonadotropin, (HCG) (n=9). Eleven additional cows, Experiment II, were ovariectomized (ovx) unilaterally at 10 days pre-partum (n=4) or 4 h post-partum (n=7) to remove the only corpus luteum (CL). These ovx post-partum animals (n=7) were also injected as part of Experiment I with saline (n=2) or HCG (n=5). All CL were sliced and incubated in media alone and with LH, tritium-labeled-pregnenolone (³HP₅) or both. Blood samples were collected pre- and post-injection, Experiment I and pre- and post-surgery, Experiment II. Plasma and media samples were analyzed for progesterone (P₄) by radioimmunoassay. Media samples with ³HP₅ were chromatographed for determination of P₅ conversion to P₄. No significant differences (P > 0.1) between P₄ profiles for saline or HCG treated animals were observed. Pre- and post-partum CL slices were able to convert P₅ to P₄. Post-partum CL slices were unresponsive to LH whereas pre-partum slices responded. These data indicate the decline in luteal function peri-partum is probably not due to withdrawal of gonadotropin support.