Hexose kinases of avocado |
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| Authors: | Les Copeland Gregory J Tanner |
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| Institution: | Dept of Agricultural Chemistry, Univ. of Sydney, N.S.W. 2006, Australia;CSIRO, Division of Plant Industry, Canberra, A.C.T. 2601, Australia. |
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| Abstract: | The subcellular location and properties of enzymes concerned with the phosphorylation of glucose and fructose in avocado (Persea americana Mill. cv. Hass) have been studied. A substantial amount of glucose-phosphorylating activity was particulate and fractionation of extracts by sucrose density gradient centrifugation indicated that most of this activity was associated with the mitochondria. Three hexose-phosphorylating enzymes were resolved by DEAE-cellulose chromatography of the cytosolic fraction. These were a hexokinase (EC 2.7.1.1), which had strong preference for glucose as substrate, and two specific fructokinases (EC 2.7.1.4). ATP was the preferred phosphoryl donor for the hexose kinases of avocado. |
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| Keywords: | Avocado hexokinase fructokinase kinetic properties Persea americana subcellular location |
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