Identification and characterization of two related murine genes, Eat2a and Eat2b, encoding single SH2-domain adapters |
| |
Authors: | Silvia Calpe Erika Erd?s Gongxian Liao Ninghai Wang Svend Rietdijk Maria Simarro Beata Scholtz Jill Mooney Chang Hoon Lee Min Sun Shin Éva Rajnavölgyi John Schatzle Herbert C Morse III Cox Terhorst Arpad Lanyi |
| |
Institution: | (1) Division of Immunology BIDMC, Harvard Medical School, 77 Ave. Louis Pasteur, Boston, MA 02115, USA;(2) Institute of Immunology, University of Debrecen Medical and Health Science Center, 98 Nagyerdei Blv., Debrecen, 4012, Hungary;(3) Department of Biochemistry and Molecular Biology, University of Debrecen Medical and Health Science Center, 98 Nagyerdei Blv., Debrecen, 4012, Hungary;(4) Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, 5640 Fishers Lane, Rockville, MD 20852, USA;(5) Department of Pathology and Center for Immunology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA |
| |
Abstract: | Human EAT-2 (SH2D1B) and SLAM-associated protein (SAP) (SH2D1A) are single SH2-domain adapters, which bind to specific tyrosine
residues in the cytoplasmic tail of six signaling lymphocytic activation molecule (SLAM) (SLAMF1)-related receptors. Here
we report that, unlike in humans, the mouse and rat Eat2 genes are duplicated with an identical genomic organization. The coding regions of the mouse Eat2a and Eat2b genes share 91% identity at the nucleotide level and 84% at the protein level; similarly, segments of introns are highly
conserved. Whereas expression of mouse Eat2a mRNA was detected in multiple tissues, Eat2b was only detectable in mouse natural killer cells, CD8+ T cells, and ovaries, suggesting a very restricted tissue expression of the latter. Both the EAT-2A and EAT-2B coimmunoprecipitated
with mouse SLAM in transfected cells and augmented tyrosine phosphorylation of the cytoplasmic tail of SLAM. Both EAT-2A and
EAT-2B bind to the Src-like kinases Fyn, Hck, Lyn, Lck, and Fgr, as determined by a yeast two-hybrid assay. However, unlike
SAP, the EAT-2 proteins bind to their kinase domains and not to the SH3 domain of these kinases. Taken together, the data
suggest that both EAT-2A and EAT-2B are adapters that recruit Src kinases to SLAM family receptors using a mechanism that
is distinct from that of SAP.
Electronic supplementary material Supplementary material is available for this article at and accessible for authorised users.
S. Calpe and E. Erdős contributed equally to this work |
| |
Keywords: | Adapter proteins EAT-2 SLAM Src kinases |
本文献已被 PubMed SpringerLink 等数据库收录! |
|