| 类囊体膜生物传感器的固定化研究 |
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| 引用本文： | 贺筱蓉,俞骅,郑立明.类囊体膜生物传感器的固定化研究[J].天然产物研究与开发,2002,14(5):54-56. |
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| 作者姓名： | 贺筱蓉 俞骅 郑立明 |
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| 作者单位： | 浙江教育学院,杭州,310012 |
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| 摘 要： | 本实验以菠菜为材料 ,经过提取 ,用PUA SbQ、海藻酸钙和戊二醛—牛血清蛋白三种固定方法在 4 0孔板上固定得到固定化的类囊体膜 ,用酶标仪连续测定 8周固定化类囊体膜的光合活力变化。结果表明 :PVA SbQ固定方法最好 ,8周后测定的活性仍保持在 6 1 2 2 %左右。
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| 关 键 词： | 类囊体膜 固定 PVA-SbQ 海藻酸钙 戊二醛—牛血清蛋白 |
| 修稿时间： | 2002年2月21日 |
STUDIES ON IMMOBILIZATION OF THYLAKOID MEMBRANE AS A BIOSENSOR |
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| HE Xiao rong,YU Hua,ZHEN Li ming.STUDIES ON IMMOBILIZATION OF THYLAKOID MEMBRANE AS A BIOSENSOR[J].Natural Product Research and Development,2002,14(5):54-56. |
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| Authors: | HE Xiao rong YU Hua ZHEN Li ming |
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| Abstract: | Spinach is used as material for thylakoid membrane.Thylakoid was immobilized in PVA SbQ,calcium alginate gel and BSA glutaraldehyde at microtiter plates and its photosynthetic activities have been measured successively within eight weeks.The results indicated that the photosynthetic activity of thylakoid immobilized in PVA SbQ was the highest,even after eight weeks the activity still remained up to 61 22%. |
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| Keywords: | Thylakoid membrane Immobilization PVA SbQ alginate gel and BSA glutaraldehyde |
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