The in vivo incorporation of mannose, retinol and mevalonic acid into phospholipids of hamster liver |
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Authors: | R M Barr L M De Luca |
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Institution: | Differentiation Control Section Lung Cancer Branch National Cancer Institute National Institutes of Health Bethesda, Maryland 20014, USA |
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Abstract: | Hamsters were injected intraperitoneally with 14C]mannose, 14C]retinol and 3H]mevalonic acid. The livers were removed, extracted with chloroform-methanol and the lipids chromatographed on DEAE-cellulose and silicic acid. The hamster liver lipid contained a component which could be labelled with mannose and mevalonic acid. The properties of this compound were in accord with it being dolichyl-mannosyl-phosphate, a possible lipid intermediate required for the biosynthesis of some glycoproteins. 14C]Retinol and 14C] mannose were incorporated into another phospholipid which was labile to mild alkali conditions commonly used for the preparation of dolichyl-mannosyl-phosphate. The retinol labelled compound had similar properties to prepared mannosyl-retinyl-phosphate. |
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Keywords: | MVA Mevalonic Acid DMP dolichyl-mannosyl-phosphate MRP mannosyl-retinyl-phosphate C:M 8:1 chloroform: methanol solution vol: vol ratio |
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