On the regulation of L-hydroxyproline dissimilatory pathway inPseudomonas aeruginosa PAO

The enzymes involved in the regulation of L-hydroxyproline degradation inPseudomonas aeruginosa PAO were investigated. L-hydroxyproline when present in the growth medium induces all the four enzymes in the pathway. Growth of the cells in L-proline also weakly induced the enzymes. The organism failed to utilize D-allo-hydroxyproline due to permeability factors. Mutants blocked in the oxidative pathway of L-hydroxyproline were isolated and enzymatically characterized. In all the mutants lacking any one of enzymes of the metabolic pathway, L-hydroxyproline is still active in inducing the remaining enzymes of the pathway suggesting that L-hydroxyproline has intrinsic inducer activity.