束缚浸水应激对大鼠肝胆汁分泌的影响及其作用机制的研究

目的：通过研究束缚浸水应激对大鼠肝胆汁分泌的影响,探讨其作用机制。方法：实验分6组（n=8）：A组：室温下单纯束缚＋生理盐水;B组：束缚浸水应激＋生理盐水;C组：室温下单纯束缚＋阿托品;D组：束缚浸水应激＋阿托品;E组：室温下单纯束缚＋酚妥拉明;F组：束缚浸水应激＋酚妥拉明。结果：与A组比较,B组肝胆汁分泌量显著减少（P〈0．05）,pH值显著升高（P〈0．01）;与A组比较,C组肝胆汁分泌量变化不明显,只有微量减少：E组与A组相对照肝胆汁分泌量无明显变化,但也有微量减少。与B组比较,D组肝胆汁分泌量有明显变化（P〈0．05）,肝胆汁pH无明显变化;F组与B组对照其肝胆汁分泌量有明显变化（P〈0．05）,肝胆汁pH无明显变化;D组与F组对照肝胆汁分泌量无明显变化,肝胆汁pH无明显变化。结论：束缚浸水应激对大鼠肝胆汁分泌有明显地抑制作用。浸水应激组肝胆汁的分泌量明显减少,肝胆汁的pH明显升高;迷走神经、交感神经在室温单纯束缚的情况下对肝胆汁的分泌影响较小,而在束缚浸水的情况下迷走神经、交感神经对肝胆汁的分泌有较显著的影响（P〈0．05）。

Studies on effects and mechanism of water immersion restraint stress on bile secretion in rats

Aim: To probe into the operation mechanism of stress,through the studies on the effects of bile secretion in rats at the condition of water immersion restraint.Methods: The animals were divided into six groups(n=8): Group A: restraint alone under room temperature+saline;Group B: water immersion restraint+saline;Group C: restraint alone under room temperature+Atropine;Group D: water immersion restraint+Atropine;Group E: restraint alone under room temperature+Phentolamine;F group: water immersion restraint+Phentolamine.Results: Compared with group A,the capacity of bile secretion in group B decreased significantly(P<0.05),changes of bile increased remarkably(P<0.01),but there were no significant decreases on the capacity of bile secretion in group C(P>0.05) compared with A,Group C only decreased appreciably.Compared with group A,the capacity of bile secretion in group E decreased appreciably(P<0.05).Compared with group B,the capacity of bile secretion in group D decreased significantly(P<0.05),pH of bile had no significant changes in group D.Compared with group B,the capacity of bile secretion in group F decreased significantly(P<0.05),pH of bile had no significant changes in group F.Compared with group D,the capacity of bile secretion and pH of bile in group F had no significant changes.Conclusion: Water immersion restraint stress inhibited evidently on the capacity of bile secretion,and the capacity of bile secretion in water immersion groups decreased significantly,moreover pH of bile increased greatly.At the condition of restraint alone under room temperature,vagus and sympathetic nerve had no significant effects on the bile secretion,but they played important roles in decreases of bile secretion evidently induced by water immersion restraint stress in rats(P<0.05).