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Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors
Authors:Jing He  Jianjun Zhu  Changguo Gong  Jiming Qi  Han Xiao  Bin Jiang  Yulan Zhao
Institution:1. School of Life Science, East China Normal University, Shanghai, PR China.; 2. Shanghai Integrated Circuit Research & Development Center, Shanghai, PR China.; 3. Ocean Research Center of Zhoushan, Zhejiang University, Zhejiang Province, PR China.; 4. Wuhan Medical and Health Center for Women and Children, Wuhan, Hubei Province, PR China.; International Centre for Genetic Engineering and Biotechnology, ITALY,
Abstract:

Background

The diagnostic and prognostic value of microRNAs (miRNAs) in a variety of diseases is promising. The novel silicon nanowire (SiNW) biosensors have advantages in molecular detection because of their high sensitivity and fast response. In this study, poly-crystalline silicon nanowire field-effect transistor (poly-SiNW FET) device was developed to achieve specific and ultrasensitive detection of miRNAs without labeling and amplification.

Methods

The poly-SiNW FET was fabricated by a top–down Complementary Metal Oxide Semiconductor (CMOS) wafer fabrication based technique. Single strand DNA (ssDNA) probe was bind to the surface of the poly-SiNW device which was silanated and aldehyde-modified. By comparing the difference of resistance value before and after ssDNA and miRNA hybridization, poly-SiNW device can be used to detect standard and real miRNA samples.

Results

Poly-SiNW device with different structures (different line width and different pitch) was applied to detect standard Let-7b sample with a detection limitation of 1 fM. One-base mismatched sequence could be distinguished meanwhile. Furthermore, these poly-SiNW arrays can detect snRNA U6 in total RNA samples extracted from HepG2 cells with a detection limitation of 0.2 μg/mL. In general, structures with pitch showed better results than those without pitch in detection of both Let-7b and snRNA U6. Moreover, structures with smaller pitch showed better detection efficacy.

Conclusion

Our findings suggest that poly-SiNW arrays could detect standard and real miRNA sample without labeling or amplification. Poly-SiNW biosensor device is promising for miRNA detection.
Keywords:
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