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Base editor screens for in situ mutational scanning at scale
Affiliation:1. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA;2. Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA;1. Howard Hughes Medical Institute, Cambridge, MA 02139, USA;2. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA;3. McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;4. Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;5. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;6. National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA;1. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA;2. Center for Mass Spectrometry, Harvard University, Cambridge, MA 02138, USA;1. Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37237, USA;2. Center for Chromosome Stability, Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen, 2200 Copenhagen, Denmark;1. Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA;2. Department of Hepatopancreatobiliary Surgery, Shenzhen Institute of Translational Medicine, Health Science Center, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, China;3. Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA;1. Department of Pathology & Immunology, Center for Genome Integrity, Washington University School of Medicine, St. Louis, MO 63110, USA;2. Center for Computational Molecular Biology, Department of Molecular Biology, Cell Biology & Biochemistry, Brown University, Providence, RI 02912, USA;3. Department of Molecular and Cellular Oncology, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA;4. Mechanisms and Regulation of Splicing Research Group, The Institute of Cancer Research, London, UK;5. Division of Oncology, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA;6. Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA;7. Department of Psychiatry, Intellectual and Developmental Disabilities Research Center, Washington University School of Medicine, St. Louis, MO 63110-1093, USA;8. Department of Biochemistry & Molecular Biophysics, Washington University in St. Louis School of Medicine, St. Louis, MO 63110, USA;9. Center for Science and Engineering of Living Systems, Washington University in St. Louis, St. Louis, MO 63130, USA;10. Hassenfeld Child Health Innovation Institute of Brown University, Providence, RI 02912, USA;1. Sir William Dunn School of Pathology, University of Oxford, Oxford, UK;2. Division of Molecular Biology, Ruđer Bošković Institute, Zagreb, Croatia;3. School of Biosciences, University of Sheffield, Sheffield, UK;4. Department of Biology, University of Oxford, Oxford, UK
Abstract:
Keywords:base editing  base editor screen  mutational scanning  CRISPR  genome editing  functional genomics  drug resistance  variant classification
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