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Type IV-A CRISPR-Csf complex: Assembly,dsDNA targeting,and CasDinG recruitment
Affiliation:1. Department of Biochemistry, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China;2. Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Department of Chemical Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China;3. Shenzhen Key Laboratory of Cell Microenvironment, Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research, Southern University of Science and Technology, Shenzhen 518055, China;4. Faculty of Pharmaceutical Sciences, Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China;5. Center for Human Tissues and Organs Degeneration, Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China;6. Key University Laboratory of Metabolism and Health of Guangdong, Southern University of Science and Technology, Shenzhen 518055, China;1. National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Institute of Molecular Physiology, Shenzhen Bay Laboratory, Shenzhen 518132, China;4. School of Medicine, Sun Yat-sen University, Shenzhen 518107, China;5. Ministry of Education (MOE) Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China;6. Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan 430072, China;1. Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland;2. Department of Medicine IV, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany;3. SIB Swiss Institute of Bioinformatics, Basel, Switzerland;4. Faculty of Natural Sciences, University of Basel, Basel, Switzerland;5. Novartis Institutes for Biomedical Research, Novartis Pharma AG, 4002 Basel, Switzerland;6. Department of Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research, Cambridge, MA, USA;1. School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China;2. Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200125, China;3. WLA Laboratories, Shanghai 201208, China;4. Shanghai Institute of Precision Medicine, Shanghai 200125, China;5. Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou 510006, China;6. Institute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai 200031, China;7. Guangzhou Medical University, Guangzhou 511436, China;8. Zhejiang Lab, Hangzhou 311121, Zhejiang, China;9. Bioinformatics Center of AMMS, Beijing 100850, China;10. Zhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, and Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou 310029, China;1. Howard Hughes Medical Institute, Cambridge, MA 02139, USA;2. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA;3. McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;4. Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;5. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;6. National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA;1. Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Abstract:
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  • Keywords:CRISPR  type IV-A CRISPR-Csf system  crRNA maturation  dsDNA targeting  PAM recognition  R-loop formation  CasDinG recruitment  helicase activity  genome engineering  bacterial defense systems
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