Molecular analysis of NlugCSP3 with behaviorally active ligands of the brown planthopper,Nilaparvata lugens (Hemiptera: Delphacidae) |
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| Affiliation: | 1. Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;2. Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China;3. Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, National Demonstration Center for Experimental Plant Science Education, Agricultural College of Guangxi University, Nanning 530004, China;1. Department of Entomology, University of California, Riverside, CA, USA;2. Laboratory of Insect Natural Enemies, Faculty of Agriculture, Kyushu University, Motooka, Nishi-ku, Fukuoka, Japan;3. Laboratory of Applied Entomology, University of Miyazaki, Miyazaki, Japan;1. The Hokkaido University Museum, Hokkaido University, Kita 10, Nishi 8, Kita-ku, Sapporo 060-0810, Japan;2. Negaunee Integrative Research Center, Field Museum of Natural History, 1400 S Lake Shore Drive, Chicago, IL 60605, USA;1. Departamento de Control Biologico (= CNRCB), SENASICA. Km 1.5 Carr. Tecoman-Estacion FFCC. C.P. 28110. Tecoman Colima, Mexico;2. Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima. Km 40 Aut. Colima-Manzanillo. C.P. 28930. Tecoman Colima, Mexico;3. Biobest Mexico S.A. de C.V. N°24, Josefa Ortiz de Domínguez, C.P. 45235, Zapopan, Jalisco, Mexico;1. Institute of Zoology, University of the Punjab, Lahore, Pakistan;2. Department of Environmental Sciences, Kohsar University, Murree 47150, Pakistan;3. Department of Entomology, University of the Punjab, Lahore, Pakistan;1. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan 410128, People''s Republic of China;2. Institute of Virology, Hunan Agricultural University, Changsha, Hunan 410128, People''s Republic of China;1. Department of Biotechnology, Bharathidasan University, Trichirappalli 620024, Tamil Nadu, India;2. Institute of Clinical Medicine, National Yang-Ming University, Taipei 112, Taiwan, Republic of China;3. Department of Zoology, HKRH College, Uthamapalayam, Theni District, Tamil Nadu, India;4. Department of Microbiology, HKRH College, Uthamapalayam, Theni District, Tamil Nadu, India;5. Department of Zoology, University of Madras, Guindy Campus Chennai, Tamil Nadu, India;6. Vice-Chancellor, Central University, Thiruvarur, Tamil Nadu, India |
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| Abstract: | The insect olfactory system is sensitive to the complicated chemical environment. Insect’s chemosensory proteins (CSPs) supposedly act as transport of plant volatiles across the sensillar lymph and competitive fluorescent binding assay was commonly used to test the binding affinities with various plant volatiles. However, extensive research to determine the physiological role of CSPs is necessary and helpful through binding interaction of a protein with the plant volatiles. In this comparative study, we employed phylogenetic analysis, fluorescence spectra, quenching mode, and thermodynamic force to characterize Nilaparvata lugens CSP3 (NlugCSP3). The phylogenetic tree revealed that amino acid sequence of NlugCSP3 showed extremely close similarities with Laodelphax striatella (LstrCSP10 & LstrCSP11) and Sogatella furcifera (SurCSP3). The Stern-Volmer (SV) curve of NlugCSP3 fluorescence quenching indicated that nonadecane and 2-tridecanone clearly quenched NlugCSP3 fluorescence as a stable static quenching mode. Meanwhile, α-terpinene and farnesene collided with NlugCSP3, instead of forming stable complexes. The thermodynamic analysis of NlugCSP3 revealed that spontaneous binding interaction occurred in nonadecane and 2-tridecanone and is driven primarily by hydrophobic interactions. This study not only provides the information about the binding interaction of protein with the volatiles, but also improves the efficient recognition of behaviorally elicited volatiles that could be used in the management of brown planthopper. |
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| Keywords: | Chemosensory proteins Phylogenetic analysis Fluorescence quenching assay Behaviorally elicited volatiles Binding interaction |
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