Secreted protease of the entomopathogenic fungus <Emphasis Type="Italic">Cordyceps militaris</Emphasis>. II. Enzyme properties and adsorption on the insect cuticle期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Secreted protease of the entomopathogenic fungus Cordyceps militaris. II. Enzyme properties and adsorption on the insect cuticle

Authors:	T. A. Semenova M. A. Belozerskii G. A. Belyakova B. A. Borisov S. A. Semenova Ya. E. Dunaevskii

Affiliation:	1.Department of Mycology and Algology, Biological Faculty,Moscow State University,Moscow,Russia;2.Belozerskii Institute of Physicochemical Biology,Moscow State University,Moscow,Russia;3.Center of Parasitology, Severtsov Institute of Ecology and Evolution,Russian Academy of Sciences,Moscow,Russia

Abstract:	Electrophoretically pure preparation of a secreted subtilisin-like protease was isolated from the culture liquid of an entomopathogenic fungus C. militaris strain KP-97-2. The enzyme had an optimum activity at 45°C and pH 7–9. Data were collected on the substrate specificity of the enzyme and its stability at different values of pH and temperature. Adsorption and desorption of the extracellular protease on the cuticle of Nauphoeta cinerea was studied in function of pH.

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