On the activity loss of hydrolases in organic solvents: II. a mechanistic study of subtilisin Carlsberg |
| |
Authors: | Betzaida Castillo Vibha Bansal Ashok Ganesan Peter Halling Francesco Secundo Amaris Ferrer Kai Griebenow Gabriel Barletta |
| |
Affiliation: | (1) Department of Chemistry, University of Puerto Rico, Rio Piedras Campus, P.O Box 23346, San Juan, 00931-3346, Puerto Rico;(2) Department of Chemistry, University of Puerto Rico at Humacao, CUH Station, Humacao, 00791, Puerto Rico;(3) WestCHEM, Department of Pure & Applied Chemistry, University of Strathclyde, Glasgow, G1 1XL, UK;(4) Istituto di Chimica del Riconoscimento Molecolare, CNR, Via Mario Bianco 9, 20131 Milano, Italy |
| |
Abstract: | Background Enzymes have been extensively used in organic solvents to catalyze a variety of transformations of biological and industrial significance. It has been generally accepted that in dry aprotic organic solvents, enzymes are kinetically trapped in their conformation due to the high-energy barrier needed for them to unfold, suggesting that in such media they should remain catalytically active for long periods. However, recent studies on a variety of enzymes demonstrate that their initial high activity is severely reduced after exposure to organic solvents for several hours. It was speculated that this could be due to structural perturbations, changes of the enzyme's pH memory, enzyme aggregation, or dehydration due to water removal by the solvents. Herein, we systematically study the possible causes for this undesirable activity loss in 1,4-dioxane. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|