High resolution ultrasound-guided microinjection for interventional studies of early embryonic and placental development in vivo in mice |
| |
| Authors: | John C Slevin Lois Byers Marina Gertsenstein Dawei Qu Junwu Mu Nana Sunn John CP Kingdom Janet Rossant S Lee Adamson |
| |
| Affiliation: | (1) Samuel Lunenfeld Research Institute of Mount Sinai Hospital, Toronto, Canada;(2) Department of Obstetrics and Gynecology, University of Toronto, Toronto, Canada;(3) Department of Physiology, University of Toronto, Toronto, Canada;(4) Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Canada |
| |
| Abstract: | Background In utero microinjection has proven valuable for exploring the developmental consequences of altering gene expression, and for studying cell lineage or migration during the latter half of embryonic mouse development (from embryonic day 9.5 of gestation (E9.5)). In the current study, we use ultrasound guidance to accurately target microinjections in the conceptus at E6.5–E7.5, which is prior to cardiovascular or placental dependence. This method may be useful for determining the developmental effects of targeted genetic or cellular interventions at critical stages of placentation, gastrulation, axis formation, and neural tube closure. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
