首页 | 本学科首页   官方微博 | 高级检索  
     


Multi-photon excitation microscopy
Authors:Alberto Diaspro  Paolo Bianchini  Giuseppe Vicidomini  Mario Faretta  Paola Ramoino  Cesare Usai
Affiliation:(1) LAMBS-MicroScoBio Research Center, Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genova, Italy;(2) IFOM,The FIRC Institute for Molecular Oncology Foundation, Via Adamello 16, 20139 Milan, Italy;(3) IFOM-IEO Consortium for Oncogenomics European Institute of Oncology, via Ripamonti 435, 20141 Milan, Italy;(4) DIPTERIS - Department for the Study of the Territory and its Resources, University of Genoa, Corso Europa 26, 16132 Genova, Italy;(5) CNR- National Research Council, Institute of Biophysics, Via De Marini, 6, 16149 Genova, Italy
Abstract:Multi-photon excitation (MPE) microscopy plays a growing role among microscopical techniques utilized for studying biological matter. In conjunction with confocal microscopy it can be considered the imaging workhorse of life science laboratories. Its roots can be found in a fundamental work written by Maria Goeppert Mayer more than 70 years ago. Nowadays, 2PE and MPE microscopes are expected to increase their impact in areas such biotechnology, neurobiology, embryology, tissue engineering, materials science where imaging can be coupled to the possibility of using the microscopes in an active way, too. As well, 2PE implementations in noninvasive optical bioscopy or laser-based treatments point out to the relevance in clinical applications. Here we report about some basic aspects related to the phenomenon, implications in three-dimensional imaging microscopy, practical aspects related to design and realization of MPE microscopes, and we only give a list of potential applications and variations on the theme in order to offer a starting point for advancing new applications and developments.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号