Fast, easy and efficient: site-specific insertion of transgenes into Enterobacterial chromosomes using Tn7 without need for selection of the insertion event |
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| Authors: | Gregory J McKenzie Nancy L Craig |
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| Institution: | (1) Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 21205 Baltimore, Maryland, USA |
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| Abstract: | Background Inserting transgenes into bacterial chromosomes is generally quite involved, requiring a selection for cells carrying the
insertion, usually for drug-resistance, or multiple cumbersome manipulations, or both. Several approaches use phage λ red recombination, which allows for the possibility of mutagenesis of the transgene during a PCR step. |
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