Characterization ofgltA: luxCDABE fusion inEscherichia coli as a toxicity biosensor |
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| Authors: | Joo-Myung Ahn Byoung Chan Kim Man Bock Gu |
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| Institution: | (1) Department of Food Engineering and Biotechnology, Technion-Israel Institute of Technology, 32000 Haifa, Israel;(2) Department of Chemistry, Haifa, Israel;(3) The Interdepartmental Program in Biotechnology, Haifa, Israel;(4) Institute of Catalysis Science and Technology, Haifa, Israel; |
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| Abstract: | The use ofgltA gene, as a new biomarker for environmental stress biomonitoring, was investigated because of its key position as the first
enzyme of the tricarboxylic acid (TCA) cycle. A recombinant bioluminescentEscherichia coli strain, EBJM2, was constructed using a plasmid carrying the citrate synthase (gltA) promoter transcribing thePhotorhabdus luminescens luxCDABE genes (gltA::luxCDABE). The responses from this strain were studied with five different classes of toxicants: DNA damage chemicals, phenolics,
oxidative-stress chemicals, PAHs, and organic solvents. EBJM2 responded strongly to DNA damage chemicals, such as mitomycin
C (MMC) and methyl-nitro-nitrosoguanidine (MNNG), and nalidixic acid with the strongest responses. In contrast, tests with
several compounds from the other four classes of toxicants gave no significant response. Therefore, EBJM2 was found to be
sensitive to DNA damage chemicals. |
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