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Short-term sequence evolution and vertical inheritance of the Naegleria twin-ribozyme group I intron |
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| Authors: | Odd-Gunnar Wikmark Christer Einvik Johan F De Jonckheere Steinar D Johansen |
| Affiliation: | 1. Department of Molecular Biotechnology, RNA Research Group, Institute of Medical Biology, University of Troms?, N-9037, Troms?, Norway 2. Department of Pediatrics, University Hospital of North Norway, N-9038, Troms?, Norway 3. Protozoology Laboratory, Scientific Institute Public Health, B1050, Brussels, Belgium 4. Department of Fisheries and Natural Sciences, Bod? University College, N-8049, Bod?, Norway |
| Abstract: | BackgroundRibosomal DNA of several species of the free-living Naegleria amoeba harbors an optional group I intron within the nuclear small subunit ribosomal RNA gene. The intron (Nae.S516) has a complex organization of two ribozyme domains (NaGIR1 and NaGIR2) and a homing endonuclease gene (NaHEG). NaGIR2 is responsible for intron excision, exon ligation, and full-length intron RNA circularization, reactions typical for nuclear group I intron ribozymes. NaGIR1, however, is essential for NaHEG expression by generating the 5' end of the homing endonuclease messenger RNA. Interestingly, this unusual class of ribozyme adds a lariat-cap at the mRNA. |
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