The evaluation of the factors that cause aggregation during recombinant expression in E. coli is simplified by the employment of an aggregation-sensitive reporter |
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Authors: | Tina Schultz Lucia Martinez Ario de Marco |
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Institution: | (1) EMBL Scientific Core Facilities, Meyerhofstr. 1, D-69117 Heidelberg, Germany;(2) IFOM-IEO Campus, Biochemistry Unit, via Adamello 16, I-20139 Milano, Italy |
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Abstract: | Background The yields of soluble recombinant proteins expressed in bacteria are often low due to the tendency of the heterologous proteins
to form aggregates. Therefore, aggregation reporters have been envisaged to simplify the comparison among different expression
conditions and to speed up the identification of suitable protocols that improve the solubility. The probe we used is composed
by an IbpAB promoter specifically activated by protein aggregates fused to a sequence coding the β-galactosidase, the activity of which
becomes, therefore, indicative of the aggregation degree. |
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Keywords: | |
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