Phosphorylation of anti-HIV nucleoside analogs by nucleoside diphosphate kinase. |
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| Authors: | B Schneider Y Xu O Sellam R Sarfati J Janin M Véron D Deville-Bonne |
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| Institution: | Unité de Régulation Enzymatique des Activités Cellulaires, CNRS URA1773, Institut Pasteur, Paris, France. |
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| Abstract: | The reaction of NDP kinase with antiviral nucleoside triphosphates used in antiviral therapies was studied at the presteady state by fluorescence stopped-flow and compared with the steady-state parameters. The affinity of the analogs was determined by fluorescence titration of a mutated enzyme with an inserted Trp in the binding site. The lack of the 3' hydroxyl in analogs is shown to decrease the kcat more than the KD. |
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