| 重金属铅对鲫鱼乳酸脱氢酶和过氧化氢酶活性的影响 |
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| 引用本文： | 成嘉,符贵红,刘芳,唐建洲,李基光,张建社.重金属铅对鲫鱼乳酸脱氢酶和过氧化氢酶活性的影响[J].生命科学研究,2006,10(4):372-376. |
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| 作者姓名： | 成嘉 符贵红 刘芳 唐建洲 李基光 张建社 |
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| 作者单位： | 1. 长沙学院,生物工程与环境科学系,中国湖南,长沙,410003
2. 湖南农业大学,动物科技学院,中国湖南,长沙,410128 |
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| 基金项目： | 国家自然科学基金资助项目(30571414)，湖南省自然科学基金资助项目(05JJ30151)，长沙市科技局重点创业基金资助项目(K051127-72) |
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| 摘 要： | 在实验室条件下,采用毒性实验方法研究不同浓度重金属铅(Pb2 )对鲫鱼血清乳酸脱氢酶(LDH)和血液过氧化氢酶(CAT)活性的影响.研究结果表明,随着金属离子铅浓度的增高血清中乳酸脱氢酶和过氧化氢酶活性下降,但两种酶对铅离子影响的敏感程度不同.影响LDH活性的最低铅离子浓度为0.1mmol/L,而CAT为0.5mmol/L,当Pb2 浓度为1mmol/L时,LDH活性降至10%左右,而CAT仅下降50%左右.对重金属离子浓度与酶活性的定性和定量分析为有效监控鱼类生存环境提供了有价值的参考资料.
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| 关 键 词： | 铅 鲫鱼 乳酸脱氢酶 过氧化氢酶 |
| 文章编号： | 1007-7847(2006)04-0372-05 |
| 收稿时间： | 2006-08-03 |
| 修稿时间： | 2006-09-08 |
Effects of the Heavy Metal Lead(Pb2+) on Activitiy of LDH and CAT in the Black Carp, Carassius auratus |
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| CHENG Jia,FU Gui-hong,LIU Fang,TANG Jian-zhou,LI Ji-guang,ZHANG Jian-she.Effects of the Heavy Metal Lead(Pb2+) on Activitiy of LDH and CAT in the Black Carp, Carassius auratus[J].Life Science Research,2006,10(4):372-376. |
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| Authors: | CHENG Jia FU Gui-hong LIU Fang TANG Jian-zhou LI Ji-guang ZHANG Jian-she |
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| Institution: | 1. Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, Hunan, China; 2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, Hunan, China |
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| Abstract: | Effects of different amount of heavy metal ions,lead(Pb2 ) on the activities of lactate dehydrogenase(LDH) and catalase(CAT) of Carassius auratus serum are studied by using toxicity testing method.At laboratory condition,it was confirmed that with the increase of lead(Pb2 ) ions,the activities of both LDH and CAT were decreased basically proportional to the Pb concentrations.However,the sensitivity of LDH and CAT to Pb treatment was various.The minimum concentration for LDH is about 0.1 mmol/L and the lethal concentration is 1 mmol/L.However,the CAT enzyme activity is less sensitive to Pb treatment.The amount of each enzyme was proportional to the serum volume.The results provided useful references for an efficient environment control in heavy metal pollution in aquaculture. |
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| Keywords: | lead Carassius auratus lactate dehydrogenase catalase |
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