Population balance between producing and nonproducing hybridoma clones is very sensitive to serum level, state of inoculum, and medium composition |
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Authors: | Chuck A S Palsson B O |
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Affiliation: | Cellular Biotechnology Laboratory, Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109. |
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Abstract: | Secreting and nonsecreting hybridoma populations derived from the murine hybridoma cell line 167.4G5.3 were each grown in batch culture in low serum and serum-free media. Under serum-free conditions, a secreting population gained on a predominantly nonsecreting population and competed with the existing antibody-deficient cells effectively. It was found that this competition was sensitive to state of inoculum and medium composition. We conclude that the competition between a secreting and nonsecreting, or more generally, a producing and nonproducing, population is important; the appearance of the latter may not be a significant setback in terms of expected product titer. |
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Keywords: | hybridoma nonproducer instability antibody secretion rate flow cytometer |
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