'Signature sets', minimal fragment sets for identifying protein disulfide structures with cyanylation-based mass mapping methodology |
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Authors: | Wu Wei Huang Wei Qi Jianfeng Chou Yi-Te Torng Eric Watson J Throck |
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Affiliation: | Departments of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA. |
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Abstract: | Our cyanylation (CN)-based methodology for determining disulfide structure of cystinyl proteins overcomes the limitations of conventional proteolytic methods. However, the CN-based method has the potential drawback that occasionally some CN-induced cleavage fragments may not be detected. We show that CN-based methods can overcome the failure to detect fragments by demonstrating the existence of small 'signature sets' of fragments. The link between signature sets and the robustness of CN-based methodology is validated by two case studies. |
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