大鼠支气管肺白念珠菌感染后血清IL-17水平及意义

目的探讨大鼠支气管肺白念珠菌感染后血清白细胞介素17(IL-17)的变化及意义。方法将24只Sprague Dawley(以下简称SD)健康雄性鼠随机分为3组,每组8只,免疫低下+白念珠菌感染组(阳性对照组);免疫低下+生理盐水组(阴性对照组);免疫正常+白念珠菌感染组(实验组)。因为实验过程中存在大鼠死亡,最终每组各有6只大鼠完成实验。系统应用糖皮质激素建立免疫低下的大鼠模型;实验组及阳性对照组使用标准白念珠菌菌株混悬液经气管插管注入而建立感染模型;阴性对照组使用生理盐水作对照。感染后第1、3、5、7天使用尾静脉取血分离血清;酶联免疫吸附法(ELISA)检测血清IL-17水平;第7天取血后处死大鼠,取右肺下叶组织进行HE染色观察。结果第3、5天实验组血清IL-17水平高于对照组,而第1、7天3组间无差异;两对照组IL-17水平无差异。肺组织病理:实验组呈明显炎症反应;阳性对照组呈轻微炎症反应;阴性对照组未见明显炎性细胞浸润。结论大鼠白念珠菌支气管肺感染后,免疫正常的大鼠在感染中期血清IL-17水平升高,IL-17可能通过促进中性粒细胞在气道的聚集而参与炎症反应;IL-17的水平与机体炎症反应呈正相关;IL-17在气道白念珠菌感染后炎症介导及机体防御机制的调动中起一定作用。

Serum interleukin-17 after bronchopulmonary infection by Candida albicans in rats

Objective To investigate the changes and significance of serum interleukin 17 （IL-17） in bronchiopulmonary infection of Candida albicans in rats.Methods Immunocompromised rat model was set up with glucocorticoid injection.Model rats were administered with Candida albicans suspension and salines through tracheal intubation as positive group and negative control group.Candida albicans suspension was also administered to healthy rats as experimental group.Blood was collected bia the tail vein 1,3,5,7 days after infection for serum IL-17 detection by ELISA; Rats were sacrificed after 7 days and the right lower lung tissue was removed for HE staining.Results The serum levels of IL-17 in the experimental group at day 3 and 5 were higher than those in the control group.But no significant difference was observed at day 1 and 7 among the three groups （P0.05）.Pathology showed significant inflammation of lung in the experimental grou,but mild in the positive group.Inflammatory cell infiltration was not found in the control group.Conclusions IL-17 may participate in the inflammatory response by promoting neutrophil accumulation in the airway and the level is related to the body inflammation.IL-17 plays an important role in fungal infection-mediated inflammation of respiratory tract.