Distribution of polymorphic forms at the porcine GH locus in a population of day-10 pig embryos |
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Authors: | Lechniak Dorota Long Susan E Nissen Anna K |
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Affiliation: | Department of Genetics and Animal Breeding, August Cieszkowski Agricultural University of Poznań, ul. Wo?yńska 33, 60-637 Poznań, Poland. lechniak@jay.au.poznan.pl |
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Abstract: | The present study describes an analysis of genotype and allele distribution at the porcine GH locus among day-10 pig embryos. Embryos were collected post mortem from 6 crossbred (Danish Landrace x Yorkshire) sows inseminated with mixed Duroc semen and individually frozen for later analysis. After extraction, DNA was subjected to PCR amplification and restriction analysis with Msp I and Hae II enzymes. The genotype frequencies were: Msp I CD 0.17, DD 0.83; and Hae II AA 0.33, AB 0.58; and BB 0.09. The Msp I CC genotype was not found among analysed embryos. To our knowledge, this is the first report on the genotype and allele distribution at the GH locus among early pig embryos. |
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